午夜精品乱人伦小说区,高清国产av一区二区三区,99精品一区二区三区无码吞精,亚洲AV麻豆AⅤ无码电影

您好,歡迎進(jìn)入上海力敏實(shí)業(yè)有限公司網(wǎng)站!
一鍵分享網(wǎng)站到:
您現(xiàn)在的位置:首頁 >> 產(chǎn)品中心 >> 生化試劑、抗體、血清 >> 抗體/蛋白 >> 4060SPhospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體

Phospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體

  • 更新時(shí)間:  2023-07-25
  • 產(chǎn)品型號:  4060S
  • 簡單描述
  • Phospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體
    WB IP IHC-P IHC-F IF-IC F
    H M R Hm Mk Dm Z B (C) (X) (Dg) (Pg
詳細(xì)介紹

Phospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體 #4060

PhosphoSitePlus mark.

PhosphoSitePlus® protein, site, and accession data: Akt1, Akt2, Akt3

No.SizePrice
4060L300 ul ( 60 western blots )please select country
4060S100 ul ( 20 western blots )please select country
4060P40 ul ( 8 western blots )please select country
4060carrier free & custom formulation / quantity request

 

ApplicationsReactivitySensitivityMW (kDa)Isotype
W IP IHC-P IHC-F IF-IC FH M R Hm Mk Dm Z B (C) (X) (Dg) (Pg)Endogenous60Rabbit IgG

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Dm=D. melanogaster X=Xenopus Z=Zebrafish B=Bovine Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4060:

Flow, IHC / Frozen*, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

* Product-specific protocol.

Specificity / Sensitivity

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb detects endogenous levels of Akt only when phosphorylated at Ser473.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser473 of human Akt.

Western Blotting

Western Blotting

Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, and NIH/3T3 cells, serum-starved or PDGF-treated, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (left) or PTEN (138G6) Rabbit mAb #9559 (right). Note the presence of P-Akt staining in the PTEN deficient MDA-MB-468 cells.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma comparing SignalStain® Antibody Diluent #8112 (left) to TBST/5% normal goat serum (right) using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060.

 

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.

 

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded PTEN heterozygous mutant mouse endometrium using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb. (Tissue section courtesy of Dr. Sabina Signoretti, Brigham and Women's Hospital, Harvard Medical School, Boston, MA.)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded U-87MG xenograft, untreated (left) or lambda phosphatase-treated (right), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen SKOV3 xenograft using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb.

 

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, wortmannin and U0126 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, LY294002-treated (left) or insulin-treated (right), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).

Background

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).

 

  1. Franke, T.F. et al. (1997) Cell 88, 435-7.
  2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
  3. Franke, T.F. et al. (1995) Cell 81, 727-36.
  4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
  5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
  6. Jacinto, E. et al. (2006) Cell 127, 125-37.
  7. Cardone, M.H. et al. (1998) Science 282, 1318-21.
  8. Brunet, A. et al. (1999) Cell 96, 857-68.
  9. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
  10. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
  11. Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
  12. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
  13. Cross, D.A. et al. (1995) Nature 378, 785-9.
  14. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
  15. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
  16. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
  17. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  18. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
  19. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.

Application References

  • Gray, M.J. et al. (2008) J Natl Cancer Inst 100, 109-20. Applications: Western Blotting
  • Fos, C. et al. (2008) J Immunol 181, 1969-77. Applications: Western Blotting
  • Engelman, J.A. et al. (2008) Nat Med 14, 1351-6. Applications: IHC-P (paraffin)
  • Guertin, D.A. et al. (2009) Cancer Cell 15, 148-159. Applications: IHC-P (paraffin) Western Blotting
  • Allard, D. et al. (2008) J Biol Chem 283, 19739-47. Applications: IHC-P (paraffin)
  • De Raedt, T. et al. (2011) Cancer Cell 20, 400-13. Applications: Western Blotting
  • Kleiman, L.B. et al. (2011) Mol Cell 43, 723-37. Applications: Western Blotting
  • Sykes, S.M. et al. (2011) Cell 146, 697-708. Applications: Flow Cytometry
  • Cheung, R. et al. (2011) J Clin Invest 121, 4446-61. Applications: Western Blotting
  • Siemens, N. et al. (2011) J Biol Chem 286, 21612-22. Applications: Western Blotting
  • Lan, R. et al. (2012) Am J Physiol Renal Physiol , . Applications: IHC-P (paraffin)
  • Matsuura, S. et al. (2012) Blood , . Applications: Western Blotting
  • Jothi, M. et al. (2012) Cell Cycle 11, . Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

  • 4691 Akt (pan) (C67E7) Rabbit mAb
  • 2965 Phospho-Akt (Thr308) (C31E5E) Rabbit mAb
  • 8112 SignalStain® Antibody Diluent
  • 1140 Phospho-Akt (Ser473) Blocking Peptide
  • 2967 Akt1 (2H10) Mouse mAb
  • 4051 Phospho-Akt (Ser473) (587F11) Mouse mAb
  • 2964 Akt2 (5B5) Rabbit mAb
  • 3788 Akt3 (62A8) Rabbit mAb
  • 9272 Akt Antibody
  • 9916 Phospho-Akt Pathway Antibody Sampler Kit
  • 8101 SignalSlide® Phospho-Akt (Ser473) IHC Controls
  • 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
  • 7074 Anti-rabbit IgG, HRP-linked Antibody
  • 7720 Prestained Protein Marker, Broad Range (Premixed Format)
  • 7727 Biotinylated Protein Ladder Detection Pack
  • 7003 20X LumiGLO® Reagent and 20X Peroxide
  • 4071 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)
  • 4075 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
  • 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)

Phospho-Akt (Ser473) (D9E) XP®磷酸化Akt抗體產(chǎn)品詳細(xì)信息也可和選購

 

 


留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
免费无码又爽又刺激a片| 国产又粗又猛又爽又黄的视频| 极品少妇被猛得白浆直流草莓视频 | 超碰97久久国产精品牛牛| 国产成人无码免费网站| 亚洲欧美日本| jzzijzzij亚洲乱熟无码| 精品久久久久精品亚洲av| 毛茸茸性xxxx毛茸茸毛茸茸| 国产xxx农村乱另类| 丰满白嫩人妻中出无码| 国产精品久久久久久岛| 伊人色综合久久天天五月婷 | 国产羞羞视频在线观看播放| 亚洲精品成人片在线观看| 亚洲欧美精品无码一区二区三区| 久久精品免费一区二区喷潮 | 亚洲精品国精品久久99热| 国产又色又爽无遮挡免费| 国产99久久久国产精品~~牛| 成人精品视频一区二区三区尤物| 小苹果电影完整版在线观看| 无码人妻少妇久久中文字幕| 国产伦精品一区二区三区免费迷 | 99V久久综合狠狠综合久久| 狠狠人妻久久久久久综合九色| 美女又黄又免费的视频| 亚洲中文字幕无码自拍一拍五月| 狠狠色噜噜狠狠狠狠AV不卡| 99久久久无码国产精品9| 久久婷婷五月国产色综合| 一个人www在线观看免费资源| 欧美精品乱码99久久蜜桃| 亚洲色无码a片一区二小说| 在线视频国产欧美另类| 亚洲国产婷婷六月丁香| 普通话做受对白xxxxx在线| 玩两个丰满老熟女在线视频| 777午夜精品久久av蜜臀| 精品一区二区三区四区在线观看| 玩弄人妻aa性色少妇|