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EPTOMICS代理Atg12 (ATG12) antibody Eptomics公司抗體供應商

  • 更新時間:  2023-07-25
  • 產(chǎn)品型號:  3473-1
  • 簡單描述
  • EPTOMICS代理Atg12 (ATG12) antibody Eptomics公司抗體供應商
    Recommended Applications

    WB, IHC, ICC, IP, FC
詳細介紹

EPTOMICS代理Atg12 (ATG12) antibody Eptomics公司抗體供應商

Rabbit Monoclonal Antibody

 

Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy (1). It is essential for Atg12 conjugation, and the ATG12-ATG5 conjugate is necessary to form autophagic vesicles (2). The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required (3).

Recommended Applications

WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1:50-100
ICC: 1:100-250
IP: 1:10-100
FC: 1:10-100
 

Species Reactivity *

Human

*Species cross-reactivity is based on WB analysis

Products Data

 

Western blot analysis on (A) THP-1, (B) HepG2, (C) Raji, and (D) fetal kidney lysates using anti-Atg12 RabMAb (cat. #3473-1).Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 RabMAb (cat. #3473-1).Western blot analysis on (A) Atg12 transfected 293T and (B) 293T lysates using anti-Atg12 RabMAb (cat. #3473-1).

Specificity

A synthetic peptide corresponding to residues in human Atg12 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ATG12 antibody, APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Ubiquitin-like protein ATG12 antibody, Autophagy-related protein 12 antibody

Description References

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.
2. Phillips AR et al. Genetics. 2008 Mar;178(3):1339-53.
3. Williams RA et al. Autophagy. 2009 Feb;5(2):159-72.

 

 

 

 

 

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Cell Lysis Buffer3500-1
Goat Anti-Rabbit IgG HRP Antibody3056-1
Goat Anti-Rabbit IgG DyLight® 4053063-1
Goat Anti-Rabbit IgG DyLight® 4883064-1
Goat Anti-Rabbit IgG DyLight® 5503065-1
Goat Anti-Rabbit IgG DyLight® 5943066-1
Goat Anti-Rabbit IgG DyLight® 6333067-1
Goat Anti-Rabbit IgG DyLight® 6503068-1

 

EPTOMICS代理Atg12 (ATG12) antibody Eptomics公司抗體供應商 

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